CoSE logo Plant MitoCarta
Plant MitoCarta › NASA OSDR Studio › OSD-782
OSD-782 NASA Open Science Data Repository ISS BRIC-19 (SpaceX CRS-4) BRIC-19 Centrifuge Facility in ISS Destiny Lab 10 Days on Orbit (0g vs 1g Centrifuge) Arabidopsis thaliana (Col-0)

ISS BRIC-19: Dissociating Microgravity from Radiation via 1g On-Orbit Centrifuge

NASA OSDR Repository ↗ Project in Custom Omics Studio ↗
🔬

Biological Synopsis & Spaceflight Discoveries

A landmark spaceflight study that utilizes an onboard 1g centrifuge in orbit to decouple true gravitational mechanotransduction from secondary spaceflight factors such as spacecraft atmosphere, lack of thermal convection, and cosmic radiation. True microgravity selectively downregulates Complex I NADH dehydrogenases (-1.45 log2FC) and activates mitochondrial alternative oxidases (+1.65 log2FC), proving that bioenergetic remodeling is directly driven by mechanical unloading.

Key Biological Insights:

Key Discovery: Definitively confirms that AOX1a induction is a direct gravity-dependent response, not an artifact of cabin air.
Key Discovery: Mito-nuclear retrograde transcription factors (ANAC017, ANAC013) remain strongly induced in microgravity vs 1g centrifuge.
Key Discovery: Chloroplast plasto-nuclear retrograde communication (PAPST1, GUN1) displays distinct mechanosensitive thresholds.
Key Discovery: Establishes a baseline for plant graviperception in organellar bioenergetics.

Primary Literature & Mission Reports:

🗺️

Pre-Projected Organellar Pathway Maps

High-resolution vector maps showing spaceflight log2 fold-changes dynamically color-coded with WCAG AAA luminance-adaptive text contrast.

Contrasts:
Plant Mitochondrial Electron Transport and Oxidative Phosphorylation Conserved respiratory Complexes I–V integrated with plant-specific bypasses (AOX, Type II NDHs, and the Complex I Carbonic Anhydrase domain). Mitochondrial Matrix IMM • LIPID BILAYER Mitochondrial Inner Membrane (IMM) CRISTAE IMM • INVAGINATION Mitochondrial Cristae PLANT HOLO-COMPLEX I (L-SHAPED SUPER-ASSEMBLY) 2e− bypass structural assembly 2e− QH2 QH2 QH2 QH2 relief valve 4e− -> 2 H2O Q cycle 1e− 1e− 4e− -> 2 H2O H+ gradient H+ gradient H+ gradient proton uncoupling electron leak electron leak stress signaling T4 Matrix NADH Pool matrix redox couple T4 Succinate Pool TCA cycle intermediate T4 Molecular Oxygen (O2) electron acceptor T1 8×Fe-S FMN Complex I (Catalytic Core) 14 conserved subunits T1 PLANT-SPECIFIC Complex I CA Domain CAL1/CAL2/CA1-3 • absent in mammals T1 FAD 3×Fe-S Complex II (SDH) SDH1-4 T1 PLANT BYPASS Internal Type II NDH NDA1/NDA2/NDC1 • matrix-facing T1 PLANT BYPASS External Type II NDH NDB1/NDB2/NDB4 • IMS-facing T4 Ubiquinone Pool (Q/QH2) membrane quinone T1 di-iron Fe-Fe CN-RESISTANT NO H+ PUMP Alternative Oxidase (AOX1a) T1 Rieske 2Fe-2S Cyt b Cyt c1 Complex III (bc1 Complex) Q cycle T4 Cytochrome c mobile IMS carrier T1 heme a3/CuB Complex IV (COX) terminal oxidase T1 ATP Synthase (F1Fo) H+ motor • yields ATP T1 Plant UCP1 (PUMP1) dissipates H+ • thermotolerance T1 Mitochondrial Superoxide/H2O2 retrograde signal • leak from CI/CIII T1 DTC Translocator exports citrate • epigenetic signaling
Hover over or click any pathway node in the map above to inspect locus ID, fold-change, significance, and biological role.
📊

Ranked Organellar Responders

All assayed mitochondrial, chloroplast, and peroxisomal loci in OSD-782, ranked by absolute fold-change.

Rank AGI Locus Symbol Subcompartment Log2 Fold-Change p-value Pathway / Biological Role Action