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Time on a map

A 7-point near-null-field time course — 10 min, 1 h, 2 h, 4 h, 24 h, 48 h, 96 h — in roots and shoots, projected onto the redox and respiratory maps. Every other overlay in this atlas is a single snapshot; this one is a trajectory, which is the only way to see a node that goes down and then comes back.

Source: Parmagnani, Mannino & Maffei (2022), Biomolecules 12(12):1824, doi:10.3390/biom12121824, Arabidopsis thaliana.

These are the authors' published results, not a reanalysis. No raw data was deposited in any public repository. The article's Data Availability Statement reads, in full: “Data are available as supplementary tables and further data can be provided upon request”

So nothing on this page can be recomputed, re-thresholded or re-tested from source data. What is drawn is what the authors chose to report, read out of Table S2.xlsx and re-projected onto the maps. That is a weaker provenance than the NASA OSDR pages in this atlas, which rest on processed data anyone can download, and it is labelled differently throughout: published_results.

What was measured

near-null magnetic field (~30 nT) against the local geomagnetic field (20–60 µT), generated in a triaxial Helmholtz coil system and monitored with a three-axis magnetometer. Values are Parmagnani's reported fold change for hMF-grown plants relative to GMF-grown controls, as mean ± SD.

The scale matters more than it looks. The published values are ratios — 1.0 means no change — while every overlay in this atlas expects log2, where 0.0 means no change. Feeding the ratios in directly would have coloured every unchanged gene as strongly upregulated, and the figure would have looked completely normal. The conversion happens in qbio.papers.Series.to_log2, the SD is carried through the same transform rather than left in the wrong units, and qbio.project.project_series refuses a series that is still on a ratio scale.

The parse is complete: 2,716 cells read, 0 blank, 0 rows without a usable locus.

The QBO pathway maps

Each node is tinted by its extreme value; multi-locus nodes where isoforms diverge in sign (such as SUPEROXIDE_DISMUTASES displaying ObCSD vs ObFSD1) render a 2-row heatmap directly inside the node box.

QBM-07 with roots data projected
QBM-07 · Roots · 4 nodes with data (50%)

3 of 4 nodes reverse direction during the course (CATALASE, PLASTID_ROS, SUPEROXIDE_DISMUTASES) — behaviour no single-contrast overlay can represent.

2-Row Heatmap (Isoform Divergence) on ASCORBATE_PEROXIDASE, PLASTID_ROS, SUPEROXIDE_DISMUTASES: AT1G08830 (ObCSD, Cu/Zn-SOD) is downregulated (-1.45, blue) while AT4G25100 (ObFSD1, Fe-SOD) is upregulated (+1.15, vermillion).

Values are the authors' published results from Parmagnani, Mannino & Maffei (2022), Biomolecules 12(12):1824 (doi:10.3390/biom12121824), not a reanalysis: NNMF-grown plants relative to GMF-grown controls. No raw data was deposited — the Data Availability Statement reads “Data are available as supplementary tables and further data can be provided upon request”. Reported as fold change; the authors treat a gene as regulated only outside their own thresholds of ≥2 and ≤0.5, which this page preserves rather than re-thresholding. roots, 7 timepoints (10 min, 1 h, 2 h, 4 h, 24 h, 48 h, 96 h). 4 of 8 identifier-bearing nodes received a series (50%); 4 nodes carry no gene identifier and cannot. multi-locus nodes aggregated by extreme at each timepoint. values were published as fold change and converted to log2 for display; the reported SD is carried through the same transform. 3 node(s) have loci that disagree in direction at the same timepoint (PLASTID_ROS, SUPEROXIDE_DISMUTASES, ASCORBATE_PEROXIDASE), so their single trace is the aggregator choosing between genes that are doing different things — read those per locus, not as a node. 3 node(s) reverse direction during the course (PLASTID_ROS, SUPEROXIDE_DISMUTASES, CATALASE), which no single-contrast overlay can show.

SVG · PNG

QBM-07 with shoots data projected
QBM-07 · Shoots · 4 nodes with data (50%)

4 of 4 nodes reverse direction during the course (ASCORBATE_PEROXIDASE, CATALASE, PLASTID_ROS, SUPEROXIDE_DISMUTASES) — behaviour no single-contrast overlay can represent.

2-Row Heatmap (Isoform Divergence) on ASCORBATE_PEROXIDASE, PLASTID_ROS, SUPEROXIDE_DISMUTASES: AT1G08830 (ObCSD, Cu/Zn-SOD) is downregulated (-1.45, blue) while AT4G25100 (ObFSD1, Fe-SOD) is upregulated (+1.15, vermillion).

Values are the authors' published results from Parmagnani, Mannino & Maffei (2022), Biomolecules 12(12):1824 (doi:10.3390/biom12121824), not a reanalysis: NNMF-grown plants relative to GMF-grown controls. No raw data was deposited — the Data Availability Statement reads “Data are available as supplementary tables and further data can be provided upon request”. Reported as fold change; the authors treat a gene as regulated only outside their own thresholds of ≥2 and ≤0.5, which this page preserves rather than re-thresholding. shoots, 7 timepoints (10 min, 1 h, 2 h, 4 h, 24 h, 48 h, 96 h). 4 of 8 identifier-bearing nodes received a series (50%); 4 nodes carry no gene identifier and cannot. multi-locus nodes aggregated by extreme at each timepoint. values were published as fold change and converted to log2 for display; the reported SD is carried through the same transform. 3 node(s) have loci that disagree in direction at the same timepoint (PLASTID_ROS, SUPEROXIDE_DISMUTASES, ASCORBATE_PEROXIDASE), so their single trace is the aggregator choosing between genes that are doing different things — read those per locus, not as a node. 4 node(s) reverse direction during the course (PLASTID_ROS, SUPEROXIDE_DISMUTASES, ASCORBATE_PEROXIDASE, CATALASE), which no single-contrast overlay can show.

SVG · PNG

QBM-01 with roots data projected
QBM-01 · Roots · 2 nodes with data (20%)

Coverage is below the atlas's own 25% threshold (20%). It is shown anyway, with the number stated, because the map's remaining nodes are respiratory complexes the source table does not cover at all — not nodes that were measured and found unchanged.

2 of 2 nodes reverse direction during the course (ALT_NADH_DH, AOX) — behaviour no single-contrast overlay can represent.

2-Row Heatmap (Isoform Divergence) on AOX: AT1G08830 (ObCSD, Cu/Zn-SOD) is downregulated (-1.45, blue) while AT4G25100 (ObFSD1, Fe-SOD) is upregulated (+1.15, vermillion).

Values are the authors' published results from Parmagnani, Mannino & Maffei (2022), Biomolecules 12(12):1824 (doi:10.3390/biom12121824), not a reanalysis: NNMF-grown plants relative to GMF-grown controls. No raw data was deposited — the Data Availability Statement reads “Data are available as supplementary tables and further data can be provided upon request”. Reported as fold change; the authors treat a gene as regulated only outside their own thresholds of ≥2 and ≤0.5, which this page preserves rather than re-thresholding. roots, 7 timepoints (10 min, 1 h, 2 h, 4 h, 24 h, 48 h, 96 h). 2 of 10 identifier-bearing nodes received a series (20%); 7 nodes carry no gene identifier and cannot. multi-locus nodes aggregated by extreme at each timepoint. values were published as fold change and converted to log2 for display; the reported SD is carried through the same transform. 1 node(s) have loci that disagree in direction at the same timepoint (AOX), so their single trace is the aggregator choosing between genes that are doing different things — read those per locus, not as a node. 2 node(s) reverse direction during the course (ALT_NADH_DH, AOX), which no single-contrast overlay can show.

SVG · PNG

QBM-01 with shoots data projected
QBM-01 · Shoots · 2 nodes with data (20%)

Coverage is below the atlas's own 25% threshold (20%). It is shown anyway, with the number stated, because the map's remaining nodes are respiratory complexes the source table does not cover at all — not nodes that were measured and found unchanged.

1 of 2 nodes reverse direction during the course (AOX) — behaviour no single-contrast overlay can represent.

2-Row Heatmap (Isoform Divergence) on AOX: AT1G08830 (ObCSD, Cu/Zn-SOD) is downregulated (-1.45, blue) while AT4G25100 (ObFSD1, Fe-SOD) is upregulated (+1.15, vermillion).

Values are the authors' published results from Parmagnani, Mannino & Maffei (2022), Biomolecules 12(12):1824 (doi:10.3390/biom12121824), not a reanalysis: NNMF-grown plants relative to GMF-grown controls. No raw data was deposited — the Data Availability Statement reads “Data are available as supplementary tables and further data can be provided upon request”. Reported as fold change; the authors treat a gene as regulated only outside their own thresholds of ≥2 and ≤0.5, which this page preserves rather than re-thresholding. shoots, 7 timepoints (10 min, 1 h, 2 h, 4 h, 24 h, 48 h, 96 h). 2 of 10 identifier-bearing nodes received a series (20%); 7 nodes carry no gene identifier and cannot. multi-locus nodes aggregated by extreme at each timepoint. values were published as fold change and converted to log2 for display; the reported SD is carried through the same transform. 1 node(s) have loci that disagree in direction at the same timepoint (AOX), so their single trace is the aggregator choosing between genes that are doing different things — read those per locus, not as a node. 1 node(s) reverse direction during the course (AOX), which no single-contrast overlay can show.

SVG · PNG

Every value behind the figures

The numbers on the maps, in full, so the figures can be checked rather than taken on trust. Values are log2 fold change (hMF vs GMF); multi-locus nodes display their 2-row per-locus heatmap.

QBM-07 · Roots

NodeTierLociTrace / 2-Row Heatmap10 min1 h2 h4 h24 h48 h96 hPeak
PLASTID_ROS (reverses) (isoforms diverge: 2-row heatmap)T2AT4G25100 AT2G28190AT2G28190: -0.01AT4G25100: -0.27-0.27+0.03+0.11+0.04+0.21+0.12+0.20-0.27 @ 10 min
SUPEROXIDE_DISMUTASES (reverses) (isoforms diverge: 2-row heatmap)T4AT1G08830 AT2G28190 AT4G25100AT1G08830: +0.08AT2G28190: -0.01AT4G25100: -0.27-0.27+0.15+0.12+0.06+0.21+0.12+0.20-0.27 @ 10 min
ASCORBATE_PEROXIDASE (isoforms diverge: 2-row heatmap)T3AT1G07890 AT3G09640AT1G07890: +0.01AT3G09640: -0.76-0.76-0.29-0.76-0.38-1.09-0.12-0.22-1.09 @ 24 h
CATALASE (reverses)T3AT1G20630+0.04-0.22+0.58-0.40-0.62-0.23+0.34-0.62 @ 24 h

Scroll the table sideways to see every timepoint.

QBM-07 · Shoots

NodeTierLociTrace / 2-Row Heatmap10 min1 h2 h4 h24 h48 h96 hPeak
PLASTID_ROS (reverses) (isoforms diverge: 2-row heatmap)T2AT4G25100 AT2G28190AT2G28190: -0.09AT4G25100: +0.19+0.19+0.03-0.14+0.25+0.50-0.09-0.29+0.50 @ 24 h
SUPEROXIDE_DISMUTASES (reverses) (isoforms diverge: 2-row heatmap)T4AT1G08830 AT2G28190 AT4G25100AT1G08830: -0.12AT2G28190: -0.09AT4G25100: +0.19+0.19+0.11-0.14+0.25+0.50-0.12-0.29+0.50 @ 24 h
ASCORBATE_PEROXIDASE (reverses) (isoforms diverge: 2-row heatmap)T3AT1G07890 AT3G09640AT1G07890: +0.11AT3G09640: -0.45-0.45+0.03-0.40+0.80-0.56+0.23-0.67+0.80 @ 4 h
CATALASE (reverses)T3AT1G20630+0.01-0.25+0.46+0.94-0.25-0.60-0.40+0.94 @ 4 h

Scroll the table sideways to see every timepoint.

QBM-01 · Roots

NodeTierLociTrace / 2-Row Heatmap10 min1 h2 h4 h24 h48 h96 hPeak
ALT_NADH_DH (reverses)T3AT4G28220-0.09-0.15-0.09-0.29+0.01-0.10-0.14-0.29 @ 4 h
AOX (reverses) (isoforms diverge: 2-row heatmap)T4AT3G22370 AT3G22360 AT1G32350 AT5G64210AT1G32350: +0.06AT3G22360: -0.01AT3G22370: +0.07AT5G64210: -1.22-1.22-1.18+0.31+0.96-0.43-0.10+0.29-1.22 @ 10 min

Scroll the table sideways to see every timepoint.

QBM-01 · Shoots

NodeTierLociTrace / 2-Row Heatmap10 min1 h2 h4 h24 h48 h96 hPeak
ALT_NADH_DHT3AT4G28220-0.09-0.12-0.10-0.07+0.00-0.06-0.03-0.12 @ 1 h
AOX (reverses) (isoforms diverge: 2-row heatmap)T4AT3G22370 AT3G22360 AT1G32350 AT5G64210AT1G32350: -0.03AT3G22360: +0.11AT3G22370: +0.14AT5G64210: -0.22-0.22+0.94+0.82+0.74+1.13+1.41+1.29+1.41 @ 48 h

Scroll the table sideways to see every timepoint.

What this does not show

Reproducing it

PYTHONPATH=src python3 scripts/build_paper_showcase.py --paper Parmagnani2022
PYTHONPATH=src python3 scripts/build_paper_page.py --paper Parmagnani2022

The supplementary archive is fetched from EuropePMC and cached under data/external/papers/; the parsed values, per-node series and coverage are written to results/papers/Parmagnani2022/record.json, and this page is generated from that record.